Freezing Injury in Onion Bulb Cells

Freezing injury in onion bulb cells: I. Evaluation of the conductivity method and analysis of ion and sugar efflux from injured cells.

Onion (Allium cepa L.) bulbs were frozen to -4 and -11 C and kept frozen for up to 12 days. After slow thawing, a 2.5-cm square from a bulb scale was transferred to 25 ml deionized H(2)O. After shaking for standard times, measurements were made on the effusate and on the effused cells. The results obtained were as follows.Even when the scale tissue was completely infiltrated, and when up to 85%...

Protoplasmic Swelling as a Symptom of Freezing Injury in Onion Bulb Cells : Its Simulation in Extracellular KCl and Prevention by Calcium.

Freezing injury, in onion bulb tissue, is known to cause enhanced K(+) efflux accompanied by a small but significant loss of Ca(2+) following incipient freezing injury and swelling of protoplasm during the postthaw secondary injury. The protoplasmic swelling of the cell is thought to be caused by the passive influx of extracellular K(+) into the cell followed by water uptake. Using outer epider...

Inheritance of Bulb Color in the Onion.

Onion-bulb formation after a single compression injury in the macrophage scavenger receptor knockout mice.

Onion-bulb (OB) formation is often encountered in acquired neuropathies such as chronic inflammatory demyelinating polyradiculoneuropathy and diabetic neuropathy and is believed to require repeated injuries to peripheral nerves. Although this suggests that remaining damaged cell membranes, including myelin debris, might trigger OB formation, the molecular mechanism remains unclear. In this stud...

BULB: Onion-Based Measuring of OSS Communities

Up to date information on the associated developer community plays a key role when a company working with open source software makes business decisions. Although methods for getting such information have been developed, decisions are often based on scarce information. In this paper a measuring model for open source communities, BULB, is introduced. BULB provides a way of collecting relevant inf...